Labster enzyme kinetics

The maximum possible velocity (Vmax) occurs when all the enzyme molecules are bound with substrate [ES] = [E] total, thus: V max = k 2 [E] total. Substituting this into the prior expression gives: V = V max [S] / (K m + [S]) This is the mathematical expression that is used to model your experimental kinetic data. It is known as the Michaelis ...

Titles: enzyme kinetic Author: Praopan Yodrabum Lab: Virtual lab by Labster for BIO 1201L-03 Introduction: To investigate the alcohol, dehydrogenase CADH). enzyme, using spectrophotometer try to determine it is kinetic parameter. This enzyme is important in the manifestation of Alcohol flush syndrome. Mission # 1: Hypothesis: Enzymes are …Related documents. Enzyme Kinetics Lab MT; Basic Chem Thermo Lab MT; Energy Surfaces Lab MT; Atomic SBohr Quantum Lab MT; Separating Mixtures Lab MT; Experiment 16 Pre-LabExplain. Saltwater solution because more solute would be on the outside, meaning it would be hypertonic. List the four main components of the cell membrane. Phospholipids- non-polar, bilayer, flexible. Proteins- transport. Carbohydrates- cell to cell communication and recognition.Labstercontext for an introduction to the fundamentals of enzyme catalysis, kinetics, bioenergetics, and metabolic regulation. The virtual lab promotes mastery of the lecture ... Labster: Students must purchase access to Labster in order to access the lab component of this course. More details on how to purchase can be found in theV m a x is the Y-value (initial rate of reaction value) at which the graph above plateaus. The substrate concentration that gives you a rate that is halfway to V m a x is called the K m , and is a useful measure of how quickly reaction rate increases with substrate concentration. K m is also a measure of an enzyme's affinity for (tendency to ...Enzyme Kinetics. the study of enzyme reaction rates. Maximum Velocity. Point at which adding substrates doesn't affect the rate of product formation. Measured in moles of product per minute. Michaelis-Menten Constant (Km) concentration of the substrate for a given enzyme that produces a rate of product formation that is half the enzyme's ...

90% of students agreed that Labster provided opportunities for additional lab practice. Undergraduate students typically require more time to complete lab experiments than provided. Teach science online with Labster's interactive virtual labs. Allowing students to experiment, study remotely, and learn in a fun, effective way.The effects of microbial transglutaminase (MTGase) cross-linking on the physicochemical characteristics of individual caseins were investigated. MTGase was used to modify three major individual caseins, namely, κ-casein (κ-CN), α S -casein (α S -CN) and β-casein (β-CN). The SDS-PAG …Reaction Kinetics Lab report virtual lab report reaction kinetics: the essentials this lab report is for you to reflect on what you completed and learned in ... You can review the "THEORY" section in the simulation or at theory.labster/ if you need help. I have previous knowledge about reaction rate, catalysts, solvent, and kinetic energy ... Enzyme Kinetics. Martee Larson, Lauren Lindsey Instructor: Samuel Adjei. Section 01. Abstract: Enzymes are biological catalysts of the many reactions that take place in the human body, as well as all other living things. These catalysts can increase the rate at which the reaction takes place, allowing more product to be created in an efficient ... A unified theory of enzyme kinetics based upon the systematic analysis of the variations of k cat, k m, and k cat /k m and the relevant dG 0 values - Possible implications in chemotherapy and ...A Virgo born August 23, symbolized by the Scales, has a mercurial, kinetic charm and a graceful bearing. Learn about August 23 birthday astrology. Advertisement Virgos born on Augu...Either it is released back to enzyme and free substrate, with rate constant k-1, or else it goes on to make product, with rate constant k 2. In a steady state approximation, the enzyme substrate complex is consumed as soon as it is formed. Again, we don't know how much free enzyme there is. We don't know how much enzyme-substrate complex we have.

The Enzyme Kinetics virtual lab from Labster teaches students to work with the Michaelis-Menten equation, which is a key mathematical formula used in this topic. Also, they will learn how to use a spectrophotometer in a safe …Labster resources. Check out get the Labster resources that canned accelerate your teaching. Blog Events Kasus Degree Webinars Podcast. News Guides Research 3D Assets. ... Learn how the make a spectrophotometer, execution an enzyme kinetics experiment, analyze data and understand different inhibition mechanisms.In the Enzyme Kinetics lab, you will access a fully equipped workbench where you able getting the Alcohol Dehydrogenase reaction and measures the product are Acetylaldehyde using a spectrophotometer. You will learn about the concept of spectrophotometry, how into prepare a master mix and how to figure dilution.Wie the changes in substrate concentration (PNPP), changes in temperature, changes in pH press the presence of an inhibitor (phosphate ions) effects the rate of reaction of the reaction between PNPP andStep 1. Conclusion: The enzyme-substrate complex was formed during the enzyme kinetics lab. View the full answer Step 2. Unlock. Step 3. Unlock. Answer. Unlock.Discover Labster's award-winning virtual lab catalog for skills training and science theory. Browse simulations in Biology, Chemistry, Physics and more. Nursing. Help Center. ... Learn how to use a spectrophotometer, perform an enzyme kinetics experiment, analyze data and understand different inhibition mechanisms. 50. Min. Chemistry. Biology.

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Discover Labster's award-winning virtual lab catalog for skills training and science theory. Browse simulations in Biology, Chemistry, Physics and more. Nursing. Help Center. ... Learn how to use a spectrophotometer, perform an enzyme kinetics experiment, analyze data and understand different inhibition mechanisms. 50. Min. Chemistry. Biology.We will compare which is higher/lower than the control. What is the overall purpose of this lab? - We want to study the kinetics of pyruvate kinase. - We want to see the effect of the increasing substrate concentration on the activation of pyruvate kinase. - We want to see what can inhibitor/activate the reaction. Study with Quizlet and ...The concepts, definitions and detailed algebra of enzyme kinetics are laid out in accurate detail. For that reason, this textbook can also serve as a handbook for enzyme kinetics for research workers in the field. The research worker will find it a useful source, which can be used for solving the daily experimental problems in the laboratory.Basic Lab Skills. Bioscience Lab Skills. Chemistry Lab Skills. Chemistry for Engineers. Medical Laboratory Technology. Biomedical Sciences. Discover Labster's award-winning virtual lab catalog for skills training and science theory. Browse simulations in Biology, Chemistry, Physics and more.Discover Labster's award-winning virtual lab catalog for skills training and science theory. Browse simulations in Biology, Chemistry, Physics and more. Nursing. Help Center. ... Learn how to use a spectrophotometer, perform an enzyme kinetics experiment, analyze data and understand different inhibition mechanisms. 50. Min. Chemistry. Biology. Make a hypothesis if applicable. Hint: The purpose is often stated in the welcome message of the simulation. In this laboratory simulation, the students will learn to understand the experimental design of enzyme kinetics and conduct experiments using the enzyme Alcohol Dehydrogenase on a wild and mutant type to learn about Alcohol Flush Syndrome.

Michaelis-Menten Kinetics is shared under a CC BY-NC-SA 4.0 license and was authored, remixed, and/or curated by LibreTexts. Two 20th century scientists, Leonor Michaelis and Maud Leonora Menten, proposed the model known as Michaelis-Menten Kinetics to account for enzymatic dynamics. The model serves to explain how an ….Enzyme. Enzymes, as specific proteins, act as catalysts, lowering activation energy and accelerating reactions. While not altering the reaction equilibrium, they enhance the rate significantly. Without enzymes, reactions still occur but at a much slower pace. Their vital role in our bodies involves specific metabolic reactions, showcasing ...Active site. The area where substrates and co-factors bind to the enzyme is called the active site. This is where the catalysis takes place. An active site often appears like a pocket, and consists of several amino acids which can have specific interactions with the substrate [1]. Figure 1 shows the active site of alcohol dehydrogenase where ...Biochemistry: Enzymes and Kinetics. 47 terms. laurenpipesss. Preview. #3. 55 terms. Koolhawk4. Preview. Study with Quizlet and memorize flashcards containing terms like What is the function of a spectrophotometer?, Which wavelength is optimal to measure NADH?, Define the absorbance value displayed on the spectrophotometer. and more.Chapter 12: Enzyme Kinetics. What is chemical kinetics? Click the card to flip 👆. aka reaction kinetics, the study of rates of chemical reactions - enzyme kinetics is concerned with reactions catalyzed by enzymes. Click the card to flip 👆. 1 / 14.Digestive enzymes are found in lysosomes and in the digestive tract, where they aid the digestion of polymeric macromolecules such as proteins. These macromolecules are broken down into smaller building blocks to facilitate their uptake by the body. Figure 1: Protein digestion. Labster.com ...In the Enzyme Kinetics simulation, you will see how catalysis increases the rate of chemical reactions, with a focus on the role of enzymes in this process. ... The "Labster Virtual Lab ...... enzyme kinetics, and surveys of mammalian physiological ... Enzyme results post-lab. 5. Feb 13 - 16. Cell ... labster. 11. Apr 3 - 6. Nervous System. Nervous system ...Since allosteric enzymes are cooperative, a sigmoidal plot of \(V_0\) versus \([S]\) results: There are distinct properties of Allosteric Enzymes that makes it different compared to other enzymes. One is that allosteric enzymes do not follow the Michaelis-Menten Kinetics. This is because allosteric enzymes have multiple active sites.ADH. Alcohol Dehydrogenase (ADH) is the enzyme that catalyzes the first step in the metabolism of alcohol in humans. ADH catalyzes the oxidation of a broad range of substrates containing hydroxyl groups, including ethanol. In the case of ethanol, the alcohol is converted into acetaldehyde, another toxic compound, which is then metabolized further.

Spectrophotometer. A spectrometer is an instrument that provides information about the intensity of radiated energy. It determines the ratio between the intensity of light emitted from an internal source and that which passes through a given solution. This ratio can be used to determine the concentration of dissolved molecules in a sample.

When the enzyme is added, the reaction starts, and the enzyme is therefore not included in the master mix. This is not a case where it is crucial to use a master mix; however, the skill is essential for many applications, and it is very useful in enzyme kinetics assays in general. Enzyme Kinetics As described by Qiu et al. [ 26 ], 20 µg of the free laccase or the immobilized laccase on M2 was applied to react with a set of virtual substrate concentration ([S]) data, without considering residual errors, which was simulated for four initial guaiacol concentrations of 1, 0.5, 0.25, and 0.125 mM in HGG with or without NaN 3 ...In this study, the enzyme alcohol dehydrogenase (ADH) is used to catalyze the conversion of ethanol (the substrate) to acetaldehyde (the product). Eight kinetic trials were carried out in a pH 9.0 buffer; only the concentration of ethanol was varied from one trial to the next. The reaction was followed spectrophotometrically, although in an ... The spectrophotometer is an unsung hero of many important discoveries in chemistry, life sciences, and beyond. In this simulation, you’ll explore the mechanics at the heart of the technique. Build your spectrophotometer. Go under the hood of the spectrophotometer and solve the puzzle of how its components fit together. Measure the amount of light that passes through a cuvette. Which wavelenght is optimal to measure NADH. 340 nm. Define the absorbance value displayed on the spectrophotometer. It is the logarithmic ratio between light falling on a the sample and passing through it, log (I0/It) We need to prepare a master mix with the reagents for each reaction.Enzyme kinetics. Introduction: Enzymes are proteins that are responsible for the catalysis of life. Enzymes are defined by DNA sequences and the structure similarity are grouped into families. The molecular function of enzymes is defined as their ability to catalyse biochemical reactions by accelerating the conversation of substrates into ...Welcome to the Enzyme Kinetics case! Introduction. ADH enzyme kinetics; Lab Theory. Enzyme. ADH; Active site; Substrate; Co-factor; Co-factor for alcohol dehydrogenase; …Investigate Alcohol Flush Syndrome by studying Alcohol Dehydrogenase kinetics. Learn how the use adenine spectrophotometer, perform an enzyme chemical experiment, analyze data and understand different inhibition devices.Labster Enzyme Kinetics Discussion Guide Questions Directions: Use the following questions to enhance the discussion section in your lab report. DO NOT COPY THE QUESTIONS. Important note: The following questions are supplemental to the discussion in your report. Do not limit yourselves by only writing your discussion based on these …

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1/2 Substrate Welcome to the Labster... Substrate Substrate - Labster Theory theory.labster.com /Substrate A substrate is a molecule upon which an enzyme acts. If we think of the enzyme as a machine in an assembly line, the substrate would be what we put into the machine to get the output we want. The substrate for alcohol dehydrogenase (ADH), the enzyme we are working within this case, is ...theory.labster.comLabster resources. Verify out all to Labster resources that can accelerate your teaching. Blog Events Case Studies Webinars Podcast. News Guides Research 3D Assets. ... Learn how to use a spectrophotometer, perform an enzyme kinetics experiment, analyzing data and understand differen repression mechanisms. A laboratory work to introduce ...Introduction to the Central Nervous System: Explore your body’s command center! Microanatomy of a Neuron: Build your own neurons! Skin Layers and Organ Anatomy: Follow a skin cell’s journey! Introduction to the Male Reproductive System: Dive inside the duct system!S6: Enzyme Kinetics Cell Molecular Biology Labster 5 Co-factor Some enzymes require "helper-molecules" for catalysis to take place. These helper-molecules are called cofactors. Cofactors are non-protein molecules that bind to the enzyme and contribute to reactions in a number of different ways. Co-factors can either be inorganic ions, such as the Zn 2+ ions required by ADH, or they can be more ...Bring the world of science into the classroom or enable students to bring learning home with Labster's virtual science lab content. No need for additional hardware or lab equipment; access these organic chemistry labs on any laptops, and spark creativity in students with this innovative and interactive way to explore science. Browse Simulations.Enzyme inhibition. Enzyme inhibitors are molecules that decrease the activity of enzymes, and knowledge about inhibitors can, for example, be used in developing drugs or in the study of biochemical pathways, because inhibitors provide a way to interfere with these pathways.Enzyme inhibitors can be either irreversible or reversible; irreversible inhibitors decrease enzymatic activity by ...The enzyme catalysis is important because without it, the reaction would be too slow for be useful for life.[1] Alkaline phosphatases are plasma membrane-bound glycoproteins. Theses enzymes are widely contributed in nature including prokaryotes and higher eukaryotes. The catalyses the hydrolysis of phosphate monoesters at basic ph values.A unified theory of enzyme kinetics based upon the systematic analysis of the variations of k cat, k m, and k cat /k m and the relevant dG 0 values - Possible implications in chemotherapy and ...Bridging the enzymatic kinetics of substrate intake and overall growth phenotypes, this work offers a mechanistic interpretation to the empirical Monod law, and demonstrates the potential of ...enzyme kinetics. The last lab simulation explored Alcohol Flush Syndrome showing that people with this syndrome had a lower tolerance level for alcohol. The symptoms that are shown in these patients were skin flushing and tachycardia with only small amounts of ingested alcohol. We have learned that numerous factors impact the kinetics of enzymes.Enzyme Kinetics. Getting. ADH enzymes kinetics; Lab Theory. Enzyme. ADH; Enabled site; Substrate; Co-factor ….

Login to access Labster's catalog of virtual lab simulations and teaching resources designed to train the next generation of scientists.Enzyme kinetics has a long history (Cornish-Bowden, 1995; Michaelis and Menten, 1913). In its beginnings, it was closely connected to the understanding of the biological basis of the chemical processes that occurred in living systems. Metabolic pathways explaining the conversion of food to products secreted by living organisms, and to chemical ...Investigate Beverage Flush Syndrome by studied Alcohol Dehydrogenase kinetics. Learn how to use one spectrophotometer, perform one enzey kinetics research, analyze data furthermore understand varied inhibition mechanisms. Apply is mushroom tyrosinase to introduce michaelis-menten enzyme kinetics till biochemistry students - PubMedView ENK Enzyme Kinetics Lab Manual.pdf from PHARMACY PCOL 48P at University of San Carlos - Talamban Campus. Virtual Lab Manual Enzyme Kinetics Synopsis In the Enzyme Kinetics Lab, you will learn ... LABSTER- ENZYMES.pdf. Solutions Available. College of Charleston. CHEM 1. Enzyme Kinetics.docx. Solutions Available. University of the ...Vmax & Kcat. Figure 5.2.1: plot of Velocity vs Substrate Concentration ( V vs. [S]). On a plot of initial velocity vs Substrate Concentration ( v vs. [S]), the maximum velocity (known as V max) is the value on the Y axis that the curve asymptotically approaches. It should be noted that the value of V max depends on the amount of enzyme used in ...Enzyme kinetics is the study of how the enzymes binds their substrate and convert them into a product. The study of enzyme kinetics becomes easy when we try ...We would like to show you a description here but the site won’t allow us.Discover Labster's award-winning virtual lab catalog for skills training and science theory. Browse simulations in Biology, Chemistry, Physics and more. Nursing. Help Center. ... Learn how to use a spectrophotometer, perform an enzyme kinetics experiment, analyze data and understand different inhibition mechanisms. 50. Min. Chemistry. Biology.Atomic Structure: Atoms and Isotopes Lab Chem 102 virtual lab report atomic structure (principles): atoms and isotopes this lab report is for you to reflect onInspection Alcohol Affluent Syndrome by studying Alcohol Dehydrogenase kinetics. Learn how to using a spectrophotometer, perform an enzyme kinetics experiment, analyze data and understand others inhibition mechanisms. Labster enzyme kinetics, Enzyme Kinetics. Enzyme kinetics involves the measurement of the rate at which chemical reactions that are catalyzed by enzymes occur. Knowledge about the kinetics of an enzyme can reveal useful ..., The modern scientists have a deep understanding of enzyme kinetics. The purpose of this laboratory report is to confirm previous findings covered in various sources of scientific literature and journals. Enzymes are often described as ‘organic catalysts which increase the rate of reaction of a bio-chemical reaction’ ( David L.Nelson etal 2008)., Enzyme Kinetics. View full description. Experience Labster for Yourself. Boost Learning with Fun. 75% of students show high engagement and improved grades with Labster. ... Labster supports a wide range of courses at the high school and university level across fields in biology, chemistry and physics. ..., Titles: enzyme kinetic Author: Praopan Yodrabum Lab: Virtual lab by Labster for BIO 1201L-03 Introduction: To investigate the alcohol, dehydrogenase CADH). enzyme, using spectrophotometer try to determine it is kinetic parameter. This enzyme is important in the manifestation of Alcohol flush syndrome. Mission # 1: Hypothesis: Enzymes are biological catalyst, so by lowering the activation ..., The enzyme kinetics module is organized in one seminar followed by two laboratory sessions (4 hr each). During the seminar, the principal concepts of basic enzyme kinetics are introduced, including transition state theory, steady state and initial velocity concepts, deduction of Michaelis–Menten equation, and enzyme inhibition., In this enzyme kinetics case, at a specific substrate concentration, a master mix, containing NAD +, N A D plus ethanol, and buffer, can be prepared. The buffer keeps the pH of the reaction mixture stable. When the enzyme is added, the reaction starts, and the enzyme is therefore not included in the master mix., Cooperativity. Explore the intriguing world of enzyme kinetics and cooperative binding. Delve into the two-step process of enzyme catalysis, the Michaelis Menten equation, and the effects of increasing substrate concentration. Understand the concept of multiple substrate binding sites and the three types of cooperativity: Positive, Negative ..., Enzymes are biological catalysts, and like all catalysts, their properties are studied by measuring their rates of reaction or kinetics. It was recognized in the late nineteenth century that enzymes and their substrates must come together to form a “complex” before a reaction can occur [].This complex, non-covalent in nature, is the …, Plan de l'étude de cas sur la cinétique enzymatique. Introduction. Module 1 : Le spectrophotomètre et le master mix. Module 2 : Le titrage du substrat et de l'enzyme. Module 3 : L'optimisation du pH et de la température. Module 4 : L'ADH sauvage et son mutant. Module 5 : L'inhibition de l'enzyme., An introduction to enzyme kinetics. Explore enzyme kinetics and how enzymes speed up reactions by lowering activation energy. Learn about the two-step process of enzyme catalysis, understand the concept of Vmax, and learn about the assumptions made in enzyme kinetics. Discover how substrate concentration and enzyme concentration can affect ..., Enzyme Kinetics. Martee Larson, Lauren Lindsey Instructor: Samuel Adjei. Section 01. Abstract: Enzymes are biological catalysts of the many reactions that take place in the human body, as well as all other living things. These catalysts can increase the rate at which the reaction takes place, allowing more product to be created in an efficient ... , Bring the world of science into the classroom or enable students to bring learning home with Labster's virtual science lab content. No need for additional hardware or lab equipment; access these chemistry labs on any laptops, and spark creativity in students with this innovative and interactive way to explore science. Browse Simulations., First, the enzyme has to bind the substrate. Second, the enzyme has to transform that substrate into the product. Third, the product must be released. A reaction progress diagram for this process looks something like this: There are some energetic considerations on this diagram that we should think about., Labster Report-11-Enzyme Kinetics; Lab Report-10-Electrophilic Addition; Labster Report-9-Organic Chemistry Introduction Learn about organic compounds; ... Labster. (n.). labster/. Download. AI Quiz. AI Quiz. Download. AI Quiz. AI Quiz. 0 0. Was this document helpful? 0 0. Save Share. Matter and Phase Changes: Distil Ethanol - Labster. Course ..., , ENK Enzyme Dynamics Lab Manual - Read online for free. Labster. Labster. ENK Enzyme Kinetics Lab Manual. Uploaded by Vanesha Anes. 0 ratings 0% found dieser document useful (0 votes) 473 views. 11 pages. Select Information click to expand document information. Device:, Study with Quizlet and memorize flashcards containing terms like Which enzyme are we finding the reaction rate for today, What kind if gas is made from cellobiose, optimal temperature for enzymes and more. ... biochem lab enzyme kinetics part 1,2,3. Flashcards. Learn. Test. Match. Flashcards. Learn. Test. Match. Created by. jjohnson4404., The Michaelis-Menten equation is the most widely known model in enzyme kinetics: Where v0 is the initial reaction rate, [S] is the substrate concentration, Km is the Michaelis constant, and Vmax is the maximum reaction rate. The Michaelis constant describes the kinetics of substrate/enzyme binding. However, its precise meaning …, Regenerative braking is a highly efficient process. Check out HowStuffWorks for information about how regenerative braking works. Advertisement ­ Every time you step on your car's ..., Consequently, enzyme kinetics will be treated with a view to analyze the rates of production in different branches of a metabolic network. The analysis is based on the sensitivity of each enzymatic reaction in a particular pathway to changes in the activity of each of the pathway enzymes and to changes in the metabolite levels at the different ..., A particular enzyme at a research facility is being studied by a group of graduate students. This enzyme has a K m value of 5.0 X 10 -6 M. The students study this enzyme with an initial substrate concentration of 0.055 M. At one minute, 7 µM of product was made. What is the amount of product produced after 5 minutes., Labster Checklist Biochemistry. High Priority-ELISA -Enzyme Kinetics -Polymerase Chain Reaction. Moderate Priority. Properties of Water; Carbohydrates: Carbohydrates: The sugars that feed us; Benedict's Test for Simple Carbohydrates; Benedict's Test: Which food samples contain reducing sugars? Fehling's Test: Which food samples contain ..., Iodine Clock Reaction. There are two reaction occurring: one in which iodine is produced (the slower reaction) and second reaction in which the iodine produced is used up (the faster reaction). -Can be detected with the starch and the formation of the blue-black complex in solution (indicates that reaction 1 is complete and reaction 2 is now ..., Step 1. Conclusion: The enzyme-substrate complex was formed during the enzyme kinetics lab. View the full answer Step 2. Unlock. Step 3. Unlock. Answer. Unlock., Chemical kinetics is the measurement of how quickly reactions occur. If changes in conditions affect the speed of reaction, we can learn something about how the reaction happens. Kinetic studies are important in understanding reactions, and they also have practical implications. For example, in industry, reactions are conducted in reactors …, Digestive enzymes are found in lysosomes and in the digestive tract, where they aid the digestion of polymeric macromolecules such as proteins. These macromolecules are broken down into smaller building blocks to facilitate their uptake by the body. Figure 1: Protein digestion. Labster.com ..., Labster resources. Check leave all the Labster resources that can accelerate yours teaching. Blog Events Kasten Studies Webinars Podcast. News Guides Research 3D Assets. ... Learn how toward uses a spectrophotometer, perform einer enzyme kinetics examination, analyze data and understand different inhibition mechanisms. Experiment 5: Enzyme ..., Labster, of enzyme activity, detail the methodology, and discuss the use of high throughput techniques for profiling large numbers of samples and providing a first step in the process of identifying potential regulatory candidates. 4.2 Enzyme Kinetics In this section, we will review the basics of enzyme kinetics and, using simple exam-, apase2.123. Instructor's Note: Students will be required to use Excel to plot absorbance data and fit a line for each substrate concentration in order to calculate initial velocity. In addition, students will graph data to make Michaelis-Menten and Lineweaver-Burke plots., 6 Biochemistry Enzyme kinetics At a time when the amount of substrate is greater than the amount of enzyme, then, the rate is the initial velocity of V i. If we plot V i as a function of [S], following observations will be made: At low [S], the initial velocity,V i, rises linearly with increasing [S]. When [S] increases, V i settle down (rectangular hyperbolais formed)., Labster's enzyme-kinetics simulation allows students to feel as if they are in a real laboratory. ... Labster's stock of simulations ranges from an entertaining romp through basic lab safety,, Michaelis-Menten derivation for simple steady-state kinetics. The Michaelis-Menten equation is a mathematical model that is used to analyze simple kinetic data.The model has certain assumptions, and as long as these assumptions are correct, it will accurately model your experimental data.